Abstract: OBJECTIVE To investigate the etiological characteristics of fungal otitis externa and explore the effect of fluconazole combined with miconazole nitrate. METHODS A total of 115 patients with fungal otitis externa (124 ears) who were treated in Provincial Clinical Medical College of Fujian Medical University were enrolled in the study and randomly divided into the study group with 56 cases and the control group with 59 cases, the study group was treated with fluconazole combined with miconazole nitrate, and the control group was treated with miconazole nitrate. The distribution of pathogens was statistically analyzed. The curative effect, levels of serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α) and incidence of adverse reactions were observed and compared between the two groups. RESULTS Among the 115 patients with fungal otitis externa, the patients who were no less than 61 years of age were dominant, followed by the patients aged between 21 and 30 years old and the patients aged no more than 20 years old; the proportion of the male patients was remarkably higher than that of the female patients. Aspergillus was the predominant pathogen isolated from the 115 patients with fungal otitis externa (124 ears), accounting for 65.32%. The total effective rate of the study group was significantly higher than that of the control group (P＜0.05). The levels of serum IL-2, IL-6 and TNF-α of the study group were significantly lower than those of the control group after the treatment (P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups. CONCLUSION Aspergillus is dominant among the pathogens isolated from the patients with fungal otitis externa. Fluconazole combined with miconazole nitrate can achieve remarkable curative effect and reduce the levels of serum inflammatory factors, with the safety high.