Abstract: OBJECTIVE To explore the value of sputum culture combined with (1,3)-β-D-glucan test (G test), galactomannan test (GM test) in early diagnosis of invasive fungal infection. METHODS A total of 305 patients with suspected invasive fungal infection who were treated in Zhengzhou Central Hospital Affiliated to Zhengzhou University from Apr 2015 to Dec 2017 were enrolled in the study and divided into the infection group and the non-infection group according to the criteria for diagnosis of invasive fungal infection. The results of sputum culture, G test and GM test were analyzed, and the value of the joint detection of the three tests in early diagnosis of invasive fungal infection was observed. RESULTS Of all the patients, 113 had invasive fungal infection, accounting for 37.05%. The sensitivity of the sputum culture was 60.18%, the G test 82.30%, the GM test 69.91%, and the joint detection 97.34%. The specificity of the sputum culture was 82.81%, the G test 85.42%, the GM test 89.06%, and the joint detection 89.58%. The sensitivity of the G test was significantly higher than that of the sputum culture, the sensitivity of the joint detection of the three tests was significantly higher than that of the single detection, and there was significant difference (P<0.05). The specificity of the joint detection of the three test was higher than that of the single detection, however, there was no difference among the three tests. CONCLUSION The sputum culture combined with G test and GM test may raise the sensitivity of diagnosis of invasive fungal infection and facilitate the early diagnosis of invasive fungal infection, with the specificity high, and it has favorable application value.