Abstract: OBJECTIVE To explore the expression of fragile histidine triad (FHIT) gene and protooncogene(C-myc)in cancer tissues of cervical cancer women complicated with high-risk human papillomavirus (HPV) infection. METHODS The patients with cervical cancer who were treated in the Affiliated Hospital of Neimenggu Medical University from May 2016 to May 2018 were chosen as the cervical cancer group, the patients who had cervical intraepithelial neoplasia (CIN) were assigned as the CIN group, and the patients who received hysterectomy due to benign uterine lesions were set as the control group. The levels of FHIT and C-myc proteins in the specimens as well as the HPV subtypes were observed. RESULTS The positive rate of FHIT was significantly lower in the CIN group and the cervical cancer group than in the control group (P<0.05), the positive rate of C-myc was significantly higher in the CIN group and the cervical cancer group than in the control group (P<0.05); the positive rate of FHIT of the cervical cancer group was significantly lower than that of the CIN group (P<0.05). The incidence rate of high-risk HPV infection of the cervical cancer group was significantly higher than that of the CIN group and the control group (P<0.05). The positive expression of FHIT in cancer tissues of the cervical cancer patients was associated with the histological differentiation degree, FIGO stage and high-risk HPV infection (P<0.05). The positive expression of C-myc was associated with the histolgical differentiation degree, pathological types, FIGO stage, lymph mode metastasis and high-risk HPV infection (P<0.05). CONCLUSION The expression of C-myc is up-regulated and the expression of FHIT is down-regulated in the cancer tissues of the cervical cancer patients complicated with high-risk HPV infection, indicating that they have certain significance in diagnosis of cervical cancer.