Abstract: OBJECTIVE To investigate the presence of disinfectant resistance genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) positive for mcr-1 gene and its resistance detection, so as to provide laboratory basis for clinical selection of suitable disinfectant. METHODS The CRKP strains were collected from hospitalized patients in a hospital from Jun. 1, 2017 to Jun. 30, 2018, and identified by MALDI-TOF MS. Antimicrobial susceptibility testing was performed by VITEK2 Compact. Mcr-1 gene and four disinfectant resistance genes(qacA/B, qacE △1-sul1, merA, tehA) were detected by PCR. RESULTS Two CRKP strains carrying the mcr-1 gene were isolated. Antimicrobial susceptibility testing showed that the two strains were resistant to polymyxin, lactam and quinolones and were only sensitive to sulfamethoxazole. Both strains carried qacE Δ1-sul1 and merA genes, whereas qacA/B and tehA genes were not detected. Meanwhile, these two strains were resistant to chlorhexidine, mercury bromine, and the 84 disinfectant. CONCLUSION Two CRKP strains carrying mcr-1 gene are resistant to common antibiotics used in clinic. In addition, the experimental strains carry disinfectant resistance genes and are resistant to the corresponding disinfectants. Consequently, appropriate disinfectants should be selected to disinfect related medical devices and hospital environments so as to control the spread of such bacteria in hospitals.