Abstract: OBJECTIVE To investigate the distribution of leucocytic toxin gene(PVL) in various strains of Staphylococcus aureus isolated from clinical specimens, so as to provide the etiological basis for nosocomial infection management and monitoring. METHODS The cefoxitin disk method and polymerase chain reaction(PCR) method were used to detect mecA and pvl genes in the 193 strains of S aureus from clinical specimens in Hanchuan People's Hospital from Jan. 2017 to Dec. 2017.The results were interpreted according to the recommended criteria of CLSI (2017 version). RESULTS From the 193 strains of S aureus, 62 strains of methicillin-resistant S aureus (MRSA) were isolated, accounting for 32.1%. There were 29 pvl-positive strains, accounting for 15%, of which 21 were MSSA, accounting for 16% (21/131) of all MSSA strains, and the other 8 strains were MRSA, accounting for 12.9% (8/62) of all MRSA strains. The chi square test results showed that the difference was not significant (χ²=0.332,P=0.570). There were 6 strains of CA-MRSA and 2 strains of HA-MRSA in pvl-positive MRSA, accounting for 37.5% and 4.3%, and the difference was significant (χ²=11.609,P=0.001). CONCLUSION The detection of pvl gene in S aureus has a positive effect on clinical treatment and prognosis. The monitoring of the number and distribution of pvl gene is a necessary means for precise control of S aureus.