Abstract: OBJECTIVE To explore the clinical features and pathogenic distribution of pyogenic liver abscess(PLA)in the region, and to provide evidence for the empirical therapy of PLA. METHODS The clinical data for 193 cases of patients with pyogenic liver abscess admitted to Hwamei Hospital Affiliated to University of Chinese Academy of Sciences from Jan. 2013 to Dec. 2017 were retrospectively collected. According to the results of bacteriology, the patients with Klebsiella pneumoniae-induced pyogenic liver abscess (KP-PLA) were included in the KP-PLA group and those with Escherichia coli-induced pyogenic liver abscess (EC-PLA) were included in the EC-PLA group. The clinical manifestations, bacterial culture and drug sensitivity of both groups were analyzed. RESULTS In 193 cases of PLA, samples were available for culture of pathogens in 160 patients,of which 76 (47.5%) showed positive results. Klebsiella pneumoniae (55, 72.4%) was the main pathogen, followed by Escherichia coli (10, 13.2%). Compared with the EC-PLA group, the proportion of patients with diabetes mellitus was significantly higher in the KP-PLA group, whereas the proportions of patients with hepatobiliary surgery and malignant tumors were significantly lower (P<0.05). The K pneumoniae strains has high drug sensitivity, and the E coli strains in 6 cases (60%) produced extended-spectrum β-lactamases(ESBLs). No carbapenem-resistant Enterobacteriaceae was found in this study. CONCLUSION Some patients with PLA have atypical clinical manifestations, the main pathogenic bacteria are K pneumoniae strains, which are sensitive to most antibiotics. The second frequent pathogen is E coli, which often produces extended-spectrum β-lactamases and has resistance to cephalosporins.