Abstract: OBJECTIVE To understand the use of antimicrobial drugs, detection of pathogenic microorganisms and incidence of nosocomial infections in hospitals at all levels in Xinjiang Production and Construction Corps Area, and to provide scientific basis for the formulation of measures to prevent and treat nosocomial infection in the region. METHODS By means of cross-sectional survey, according to the Nosocomial Infection Prevalence Investigation Program of the National Nosocomial Infection Control and Management Training Base, a unified survey was conducted for all of the patients who were hospitalized in medical institutions of Xinjiang Production and Construction Corps Area on the survey day of 2014,2016 and 2018,and the results of the survey were observed and compared. RESULTS The incidence of nosocomial infections in 2014, 2016, and 2018 was 2.24%, 1.54%, and 1.71%, respectively, with significant differences(P<0.001) and showing a downward trend. The top three departments of the infection rate were comprehensive ICU, neonatal pediatrics, and neurosurgery. The top four sites of nosocomial infections were the lower respiratory tract, upper respiratory tract, superficial incision, and urinary tract. The top three pathogens detected in hospitals were Klebsiella pneumoniae, Escherichia coli, and Acinetobacter baumannii. The average use rate of antimicrobial drugs in the three surveys was 28.02%, 24.87%, and 27.87, respectively, and the difference was significant (P<0.05). CONCLUSION The cross-sectional survey of nosocomial infection can basically reflect the existing problems in the management of nosocomial infection in Xinjiang Production and Construction Corps Area, and provide evidence for the effective monitoring of hospital targets at all levels of the XPCC area and the control of nosocomial infection.