Abstract: Candida albicans is one of the most common pathogens causing hospital-acquired infections. The formation of biofilm is an important pathogenic factor of C.albicans and can greatly enhance its resistance to drugs. The formation process of C.albicans biofilm includes adhesion, proliferation, maturation, and dispersion steps. Its structure is composed of polysaccharides, proteins and lipids, extracellular DNA and some substances in the surrounding environment. Pathogenic factors include extracellular matrix and DNA, biofilm formation, pathogenicity-related proteins, quorum sensing molecules and interactions with other microorganisms. The current biofilm prevention strategies mainly include research and development of new antibacterial drugs, monomer components of traditional Chinese medicine, modified biomedical materials and novel therapies.