Abstract: OBJECTIVE To analyze the influencing factors of nosocomial plural bacterial bloodstream infection in intensive care unit（ICU）.METHODS The clinical data of 648 patients with hospital acquired bloodstream infection in ICU of the Third Affiliated Hospital of Guizhou Medical University from January 2013 to June 2019 were retrospectively analyzed. According to whether the plural bacteria were isolated from blood culture or not, the patients were divided into plural bacteria bloodstream infection group（n=96） and non-plural bacteria bloodstream infection group（n=552）. The history characteristics and laboratory examination indexes of the two groups were compared, and the influencing factors of blood flow infection of plural bacteria in ICU patients were summarized.RESULTS The nosocomial infection rate in ICU was 14.81%（96/648）. A total of 753 strains of bacteria were detected, including 201 strains of plural bacteria and 552 strains of non-plural bacteria. The composition of fungi isolated from plural bacteria NBSI was significantly higher than that from non-plural bacteria（P＜0.05）. Among the Gram-positive bacteria, there were more Staphylococcus aureus isolated from the patients with plural bacteria NBSI（P＜0.05）. Pseudomonas aeruginosa and Acinetobacter baumannii accounted for the majority of bacteria isolated from NBSI patients infected with Gram-negative bacteria（P＜0.05）; APACHE II score, invasive diagnosis and treatment, use of glucocorticoids, replacement of antibiotic and glycosylated hemoglobin were the influencing factors of hospital acquired plural bacterial bloodstream infection in ICU patients（P＜0.05）.CONLUTION Paying attention to the treatment of severe patients, reducing the time of unnecessary stay in ICU and invasive treatment, restricting the unreasonable use of glucocorticoids, carrying out the drug sensitivity test in the early stage, and rational selection of effective antibiotics, and reducing blindly frequency and more antibiotics are the main measures to reduce the hospital acquired plural bacterial bloodstream infection in ICU.