Abstract: OBJECTIVE To understand the current situation of Central Strerile Supply Department in medical institutions in Gansu Province, and to provide scientific basis for the formulating macro-management measures.METHODS From Jan. to Dec. 2018, 60 medical institutions（51 secondary and 9 tertiary） in Gansu Province were selected for questionnaire surveys and field surveys, and the results were analyzed.RESULTS A total of 60 secondary and tertiary medical institutions have independent central strerile supply departments, accounting for 35.29% and 88.89%, respectively, and the cleansing channels account for 15.69% and 88.89%, respectively. The operating procedures for cleaning medical devices, centralized management, manual cleaning and soft water treatment system account for 31.37% and 88.89%, 9.80% and 88.89%, 94.12% and 33.33%, 7.84% and 77.78%, respectively. Wet bags account for 72.55% and 11.11%, rusty equipment account for 92.16% and 33.33%, and cloth loss and rough edges account for 82.35% and 11.11%, respectively. Inadequate protection of cleaning equipment account for 68.63% and 11.11%, respectively. There was significant difference in all the data（P＜0.05）. The facilities of three-level medical institutions are generally better than those of secondary medical institutions.CONCLUTION The current situation of the Central Strerile Supply Department of medical institutions in Gansu Province is not optimistic. The layout of most Central Strerile Supply Departments is unreasonable, the distribution rate of cleaning-related equipment is low, the quality of cleaning and packaging is not up to the standard, and the personnel training in Central Strerile Supply Department is insufficient. It is necessary to strengthen the supervision and training, improve the quality of equipment cleaning and sterilization, ensure medical safety, and to actively explore the establishment of regional Central Strerile Supply Department to ensure the quality of equipment sterilization.