Abstract: OBJECTIVE To investigate the carrying situation of multi-drug resistant bacteria in nasal cavity of student nurses and hospital medical staff and to guide proper isolation protection.METHODS Nasal swabs were collected before-and after-7 months internship of 61 student nurses who were randomly selected in PLA general hospital from Jun. 2018 to Feb. 2019. Swabs also sampled at different time-period from 115 medical staff who worked in intensive care department, respiratory ICU, emergency ICU, neurology ICU and three general wards of neurology, and 18 student nurses who had 3 months internship. Those swabs were sent for pathogen identification and drug sensitivity analysis to screen the colonization of methicillin-resistant Staphylococcus aureus（MRSA） and carbapenems-resistant Enterobacter（CRE）.RESULTS A total of 255 samples were collected, CRE was not detected and MRSA detection rate was 15.38% of medical staff; CRE was not detected and MRSA detection rate was 33.33% of student nurses; CRE was not detected and MRSA detection rate was 100.00% of student nurse after 7 months internship. The difference in MRSA detection rate was significant（P=0.001）.There were significant differences in MRSA detection rate of different personnel categories（P=0.001）.The difference in MRSA detection rate was significant of student nurse before-and after-7 months internship（P=0.012）.There was no significant difference in MRSA detection rate between medical staff in different ICU and general ward（P=0.416）.CONCLUSION MRSA carrying rate in nasal cavity of student nurses before-and after internship was higher than that of medical staff, which may be related to the hospital’s emphasis on isolation and protection. It is suggested that the newly admitted nurses should be monitored and implanted with nasal colonization bacteria, and strengthen the protection during work.