Abstract: OBJECTIVE To investigate the drug resistance of carbapenem-resistant Pseudomonas aeruginosa（CRPA） in hospital in order to guide the application of clinical antibiotic, reduce bacterial resistance and prevent the nosocomial infections.METHODS A total of 95 strains of CRPA isolated from the First Affiliated Hospital of Nanchang University from Mar. to Dec. 2017 were collected to test the drug resistance. The carrying of carbapenem-related genes and outer membrane protein genes OprD2 were detected by polymerase chain reaction（PCR）.RESULTS The resistance rates of the tested strains to imipenem and meropenem were 97.89% and 95.79%, respectively, and the lowest resistance rate to piperacillin/tazobactam was 50.53%. For amikacin, cefepime, ceftazidime and levofloxacin, the resistance rates were ranged from 51% to 60%. For aztreonam, ciprofloxacin, piperacillin, gentamicin, tobramycin and cefoperazone sulbactam, the resistance rates were all more than 60%. A total of 67（70.53%） OprD2 gene-deficient strains, 12（12.63%） bla_GES positive strains, 10（10.53%） bla_VIM positive strains were detected by PCR. The bla_IMP, bla_GIM, bla_SIM, bla_TEM, bla_PER, bla_VEB, bla_OXA-10 positive strains were not detected.CONCLUSION The situation of drug resistance of CRPA is serious, and the resistance is mainly related to the carrying of bla_GES and bla_VIM gene and the absence of OprD2 gene. Clinical monitoring of CRPA should be strengthened, and antibiotics should be rationally used to minimize bacterial resistance.