Abstract: OBJECTIVE To analyze the pulsed-field gel electrophoresis（PFGE） fingerprint typing and antibiotic resistance spectrum of isolated strains of common Salmonella serotype in Wuhan and Ningbo, so as tounderstand the epidemiological feature and traceability of Salmonella isolates between these two cities.MEHTODS A total of 114 strains of the most common Salmonellaserotype（including S. typhimurium, S. enteritidis and S. derby） were isolated and identified from the medical institutions in Wuhan and Ningbo from 2014 to 2016. The PFGE fingerprint based classification and the drug resistance spectrum based on microdilution method were carried out, and the clustering analysis of strains between cities was performed through BioNumerics 7.6 software.RESULTS According to the clustering analysis of the local strains, the S. typhimurium were divided into 13 genotypes（51.70%-100.00% similarity）,the S. enteritidis were divided into 7 genotypes（76.50%-100.00% similarity） and the S. derby were divided into 21 genotypes（52.40%-100.00% similarity）. Eech serotype was found to be derived from homologous strains withsame genetic clone（≥85.00% similarity）, but different drug resistance spectrum. In particular, four strains of S. enteritidis from Ningbo and one strain of S. enteritidis from Wuhan were identified to be the same clone（100% similarity）. Among the same kind of resistant spectrum in the two cities, drug-resistant strains reached to 35.96%, with the highest resistance incidence to ampicillin（17.54%）, and multi-drug resistant strains reached to 6.14%. Among 58 local strains of Wuhan, the highest resistance incidence was detected in S. typhimurium against tetracycline（37.50%）, S. enteritidis against piperacillin, cefazolin and ampicillin（58.33%）, and S. derby against minocycline（16.67%） respectively. Among 56 local strains of Ningbo, the highest resistance incidence was observed in both S. typhimurium and S. enteritidis against trimethoprim（43.75% and 54.55% respectively） and S. derby against nalidixic acid and ampicillin（34.48%）.CONCLUSION In terms of PFGE typing analysis, S. enteritidis exhibited relatively high homology, whereas S. typhimurium and S. derby showed relatively lower homology and large discrepancy, which may result high probability of genetic recombination and mutation. Concerning clinical treatment experiences, paracilin, cefazolin and ampicillin should be used cautiously in Wuhan and so should trimethoprim in Ningbo.