Abstract: OBJECTIVE To understand the species of yeast-like fungi causing infection in patients with acquired immunodeficiency syndrome（AIDS） and observe the distribution and drug resistance so as to provide guidance for clinical treatment. METHODS By means of retrospective survey, the species of yeast-like fungi that were isolated from submitted specimens of Yunnan Provincial Infectious Disease Hospital in 2009-20117 and the results of drug susceptibility testing for commonly used antibiotics were observed. RESULTS A total of 1 373 strains of yeast-like fungi were isolated, 348 of which were isolated from sterile body fluid, including 327（94.0%） strains of Cryptococcus neoformans, 1 025 of which were isolated from other specimens, including 769（75.0%） strains of Candida albicans.There was significant difference in the distribution of isolated species among the different age groups of patients（χ²=263.10,P＜0.001）, and the isolated species of strains were dominant among the patients aged between 31 and 40 years old and the patients aged between 41 and 50 years old.The drug resistance rate of the C. neoformans strains isolated from sterile body fluid to fluconazole was 4.3%, and the drug resistance rate to amphotericin B was 0.3%.The 1 020 strains of common species of Candida were highly resistant to azole drugs, while the drug resistance rates to amphotericin B and flucytosine were low. CONCLUSION C. neoformans and C.albicans are the predominant species of yeast-like fungi causing infection in the AIDS patients, and the drug resistance rates of the yeast-like fungi were low to amphotericin B and flucytosine, which can be chosen as the preferred drugs for treatment.