Abstract: OBJECTIVE To understand the distribution and drug resistance rates of clinical Salmonella isolates isolates in Children′s Hospital Affiliated to Zhengzhou University. METHODS According to the unified scheme, the serological typing and drug susceptibility testing were performed for the clinical isolates of Salmonella isolates from Jan 2015 to De 2017, the result of drug susceptibility testing was interpreted based on the standards of CLSI 2017, and the data were statistically analyzed by using WHONET and SPSS 21.0 software. RESULTS A total of 274 strains of Salmonella isolates were isolated from 2015 to 2017, 74.82%（205/274） of which were isolated in summer.The ratio of male children to female children was 1.4∶1.84.67%（232/274） of children were less than 3 years old.The strains were mainly isolated from the stool and blood specimens.Totally 31 serotypes were identified among the 274 strains of Salmonella isolates, 36.86%（101/274） of which were Salmonella isolates enteritidis, and 36.50%（100/274） were Salmonella typhimurium. The drug resistance rates of the Salmonella strains to the commonly used antibiotics did not change significantly, the drug resistance rates of the Salmonella strains to ampicillin and piperacillin were 84.6% and 77.3%, respectively, and the drug resistance rates to the third generation cephalosporins cefotaxime and ceftazidime were 32.5% and 25.2%, respectively.Totally 6 strains of carbapenem-resistant Salmonella isolates were isolated.The drug resistance rates of the strains isolated from the stool specimens were generally higher than those of the strains isolated from the blood specimens; the drug resistance rates of the S. enteritidis strains to β-lactams were higher than those of the S. typhimurium strains. CONCLUSION The Salmonella strains are prevalent among the children in summer and autumn and are mainly isolated from the children aged less than 3 years old.The S. enteritidis and S. typhimurium are the major serotypes.The Salmonella strains isolated from different specimens vary in the resistance rates to the commonly used antibiotics, and it is necessary to reasonably use antibiotics based on the results of serological typing and drug susceptibility testing.