Abstract: OBJECTIVE To explore the impact of serum inflammatory and immunological indexes on intestinal barrier status and clinical prognosis of the patients with sepsis. METHODS The clinical data of 82 patients with sepsis who were treated in the Affiliated Hospital of North Sichuan Medical College from Sep 2015 to Sep 2018 were retrospectively analyzed, the enrolled patients were divided into the mild and moderate sepsis group with 43 cases and the severe sepsis group with 39 cases according to acute physiology and chronic health evaluation（APACHE） II score and scoring guidelines for clinical symptoms of sepsis.The blood specimens were collected from the two groups of patients so as to determine the levels of high-mobility group box 1（HMGB1） protein, interleukin-6（IL-6）, tumor necrosis factor-α（TNF-α） and immunological indexes regulatory T cells（Treg） and helper T cells（TH） as well as diamine oxidase（DAO）, D-lactic acid（D-LAC） and intestinal fatty acid binding protein（IFABP）, which could reflect the intestinal barrier status of the patients.The correlation between the above indexes and the prognosis was observed. RESULTS The levels of HMGB1, Treg, IL-6 and TH of the mild and moderate sepsis group were respectively（23.21±4.23,ng/ml）,（3.62±1.07,%）,（86.67±21.38,pg/ml） and（1.54±0.47,%）, significantly lower than those of the severe sepsis group; the TNF-α level of the mild and moderate group was（0.36±0.05,ng/L）, significantly higher than that of the severe sepsis group（P＜0.05）.The levels of intestinal barrier function indexes DAO, D-LAC and IFABP of the mild and moderate sepsis group were respectively（17.36±1.86,mg/ml）,（0.22±0.04,mmol/ml） and（21.38±3.62,pg/L）, significantly lower than those of the severe sepsis group（P＜0.05）.The levels of HMGB1, Treg, IL-6 and TH of the survival group were respectively（24.12±4.36,ng/ml）,（3.78±1.13,%）,（89.36±25.73,pg/ml） and（1.68±0.52,%）, significantly lower than those of the death group, while the TNF-α level of the mild and moderate sepsis group was（0.38±0.07,ng/L）, significantly higher than that of the death group（P＜0.05）.Multivariate analysis showed that the treatment outcomes of the patients were associated with HMGB1, Treg, IL-6, TNF-α and TH. CONCLUSION The intestinal barrier function of the sepsis patients is associated with the serum HMGB1, Treg, IL-6, TNF-α and TH, which may influence the prognosis of the patients and have certain significance in diagnosis of innless condition.