Abstract: OBJECTIVE To investigate the infection sites, species of pathogens and influencing factors for postoperative infection in fracture patients. METHODS Totally 124 fracture patients with postoperative infection and 124 fracture patients without postoperative infection who were treated in the Chinese PLA Hospital from Dec 2016 to Dec 2018 were enrolled in the study. The infection sites of the fracture patients with postoperative infection were statistically analyzed. The specimens were collected from the infection sites, the bacterial culture and drug susceptibility testing were carried out, and the univariate analysis and multivariate logistic regression analysis were performed for influencing factors for the infection. RESULTS The patients with superficial incision infection were dominant among the 124 fracture patients with postoperative infection, accounting for 45.16%. A total of 153 strains of pathogens were isolated, of which 115 (75.16%) were gram-negative bacteria, and 38(24.84%) were gram-positive bacteria; Acinetobacter baumannii (39 strains, 25.49%) and Pseudomonas aeruginosa (36 strains, 23.53%) were the predominant species of the gram-negative bacteria; Staphylococcus aureus (28 strains, 18.30%) was dominant among the gram-positive bacteria. The drug resistance rate of the A.baumannii strains to piperacillin was the highest, while the drug resistance rate to cefoperazone was the lowest; the drug resistance rate of the P.aeruginosa strains to gentamicin was the highest, while the drug resistance rate to levofloxacin was the lowest. The drug resistance rate of the S.aureus strains to ampicillin was highest, while the drug resistance rate to linezolid was lowest. The types of fracture, number of times of surgery and operation duration were the independent influencing factors for the postoperative infection in the fracture patients (P<0.05). CONCLUSION The types of fracture, number of times of surgery and operation duration are the independent influencing factors for the postoperative infection in the fracture patients. The superficial incision is the predominant infection site of the fracture patients with postoperative infection. The gram-negative bacteria are dominant among the pathogens and show multidrug-resistant, therefore, it is necessary for the hospital to reduce the number of times of surgery, shorten the operation duration and reasonably use antibiotics so as to prevent the postoperative infection and relieve the pain and burden of the fracture patients.