Abstract: OBJECTIVE To investigate the clinical characteristics of coagulase-negative Staphylococcus（CNS） infection and observe the pharmacodynamics of vancomycin. METHODS The patients who were hospitalized and treated with vancomycin in Peking University Third Hospital from Jan 2016 to Jul 2017 were enrolled in the study and divided into the Staphylococcus aureus infection group and the CNS infection group according to the pathogens causing infections.The clinical data and effect of vancomycin were observed and compared between the two groups of patients. RESULTS Of totally 52 patients who were enrolled in the study, 82.69%（43/52） had hospital-acquired infection; there were 21 cases in the S. aureus infection group and 31 cases in the CNS infection group.The positive rates of blood culture of the CNS group was 61.3%, significantly higher than 19.0% of the S. aureus group; the serum albumin level of the CNS group was 34.0 g/L, significantly higher than 29.3 g/L of the S. aureus group（P=0.031）.The treatment course of vancomycin of the CNS group was 8.0 days, significantly shorter than 15.0 days of the S. aureus group（P=0.002）.There were no significant differences in the serum trough concentration（15.0 mg/L vs 14.6 mg/L）, clinical cure rate（77.4% vs.66.7%） and clearance rate of microorganisms（74.2% vs.66.7%） between the two groups.The sputum specimens were the most common specimens where the S.aureus strains were isolated 38.1%（8/21）, followed by the wound secretions 28.6%（6/21）; the blood specimens were the most common specimens where the CNS strains were isolated 61.3%（19/31）, followed by the sputum specimens 29.0%（9/31）.The methicillin-resistant strains accounted for 76.2% among the CNS strains, 61.1% among the S.aureus strains. CONCLUSION The patients with bloodstream infection are dominant among the patients with CNS infection, the percentage of the methicillin-resistant strains is high.Vancomycin can achieve a remarkable curative effect based on the serum trough concentration.