Abstract: OBJECTIVE To reveal the epidemic cloning characteristics of Klebsiella pneumoniae strains carrying bla_KPC-2 gene that were isolated from China and compare them with strains isolated abroad. METHODS The multilocus sequence typing（MLST） was carried out for 100 strains of K. pneumoniae carrying bla_KPC-2 that were isolated from 61 hospitals in 41 cities of China from 2012 to 2017, the foreign MLST data of K. pneumoniae strains carrying bla_KPC-2 gene were obtained by means of literature retrieval, and the minimum spanning trees were constructed by using BioNumerics software. RESULTS The 100 strains of K. pneumoniae carrying bla_KPC-2 gene were divided into 14 MLST types（STs）.The dominant type was ST11, which contained 77 strains isolated from 38 cities; the next was ST15, which contained 7 strains isolated from 6 cities; the rest of 12 types were ST147, ST1, ST65, ST23, ST25, ST37, ST258, ST395, ST412, ST449, ST502 and ST685, which contained 1-3 strains respectively.The retrieved literatures showed that the dominant type of the European and American isolates was ST258. CONCLUSION As the dominant clone, ST11 type strains had spread to most areas of the country since the ST11 K. pneumoniae carrying bla_KPC-2 was first reported in China in 2007.It is necessary to strengthen the laboratory monitoring of molecular subtypes of the strains so as to obtain systematic and comprehensive data to reveal the epidemic laws and put forward targeted prevention measures.