Abstract: OBJECTIVE To analyze the correlation between serum hepatitis B virus-deoxyribonucleic acid（HBV-DNA） load and levels of heat shock protein 70（Hsp70）, carcinoembryonic antigen（CEA） and alpha-fetoprotein（AFP） in 120 patients with hepatitis B cirrhosis. METHODS A total of 120 patients with hepatitis B cirrhosis who were admitted to the Huanggang Central Hospital between May 2016 and May 2019 were accordance with the inclusion criteria and recruited as the research subjects. The HBV-DNA load, levels of HSP70, CEA and AFP were measured by fluorescent quantitative PCR and enzyme-linked immunosorbent assay（ELISA）, respectively. Levels of serum HSP70, CEA and AFP in patients with hepatitis B cirrhosis and different HBV-DNA loads were compared. The effect of confounding factors was corrected by covariance analysis, and the correlation between serum HBV-DNA load and adjusted levels of HSP70, CEA and AFP were analyzed by Pearson correlation analysis. RESULTS In the 120 patients with hepatitis B cirrhosis, HBV-DNA load was smaller than 5 log₁₀ copies/ml in 64 cases, with an average of（3.65±0.29） log₁₀ copies/ml. The load was bigger than 5 log₁₀ copies/ml or equal in 56 cases, with an average of（5.98±0.28） log₁₀ copies/ml. HSP70 levels in patients with HBV-DNA load ＜5 log₁₀ copies/ml were significantly lower than that in patients with HBV-DNA load ≥5 log₁₀ copies/ml, whereas CEA and AFP were higher than that in patients with HBV-DNA load ≥5 log₁₀ copies/ml. The Bonferroni test showed that there were significant differences in levels of HSP70, CEA and AFP between patients with different HBV-DNA loads after correction（P＜0.05）. Pearson correlation analysis showed that there was an correlation between HBV-DNA load and HSP70, CEA and AFP（r=-0.525, 0.637, 0.409, all P＜0.01）. CONCLUSION The serum HBV-DNA load in patients with hepatitis B cirrhosis was negatively correlated with HSP70, and positively correlated with CEA and AFP.