Abstract: OBJECTIVE To explore the effect of hepatitis B virus（HBV） and hepatitis C virus（HCV） infection in the males on embryo development and pregnancy outcomes of infertility women after in vitro fertilization embryo transfer（IVF-ET） process. METHODS During the period from Feb. 2018 to Feb. 2019, 104 infertile couples with husband positive infection of HBV and HCV receiving IVF-ET in Sichuan Provincial People’s Hospital were enrolled as the positive group, and 130 couples with husband negative infection with HBV and HCV during the same period were enrolled as the negative group. The fertilization, embryo development and pregnancy outcomes were compared between the two groups. RESULTS The number of transplanted embryos, embryo score, normal fertilization rate, cleavage rate and high-quality embryo rate in the positive group were（2.83±0.80）,（8.57±0.65） points, 63.93%, 85.52% and 61.23%, respectively, and that in the negative group were（3.01±0.76）,（8.62±0.67） points, 65.89%, 87.31% and 62.79%, respectively. The difference of embryo development between the two groups was not significant. The implantation rate, clinical pregnancy rate, early abortion rate, late abortion rate and delivery rate in the positive group were 58.22%, 66.35%, 8.70%, 10.14%, 53.84%, and that in the negative group were 56.52%, 70.00%, 6.59%, 5.49%, 61.54%, respectively. There was no significant difference of pregnancy outcomes between the two groups. CONCLUSION The effect of HBV and HCV infection in the males are not significant on embryo development and pregnancy outcome in couples in IVF-ET process.