Abstract: OBJECTIVE To analyze the risk factors and change of soluble myeloid triggering receptor-1（sTREM-1）, CD64 and tumor necrosis factor-α（TNF-α） levels in patients with major burn complicated with sepsis infection. METHODS The clinical data of 115 patients with major burn in the Burn Department of Kaifeng Central Hospital from Jan 2019 to Jan 2020 were retrospectively collected. According to the incidence of sepsis infection, they were divided into the sepsis group（n=35） and the non-septic group（n=80）. The serum sTREM-1, CD64 and TNF-α levels and clinical indicators were compared between the 2 groups, and the risk factors of sepsis infection after major burn were analyzed by multivariate Logistic regression. RESULTS The hospital admission time and the first operation time of the sepsis group were significantly longer than those of the non-septic group, and the proportion of cases with inhalation injury increased significantly（P＜0.05）. Serum sTREM-1, CD64 and TNF-α levels were significantly higher in the sepsis group than in the non-septic group（P＜0.05）. Multivariate logistic regression analysis showed that sTREM-1, CD64, and TNF-α levels were elevated, the first operation time was ＞3 days, no surgery, and inhalation injuries were all independent influencing factors of major burn complicated with sepsis infection（P＜0.05）. CONCLUSION There are many risk factors for severe burns complicated with sepsis infection, so clinical intervention should be targeted to reduce the risk, sTREM-1, CD64 and TNF-α levels were predictive factors.