Abstract: OBJECTIVE To investigate the drug-susceptibility and drug-resistance of clinical bacteria isolated from one hospital in Sanya city of Hainan province. METHODS Clinical isolates from a hospital in Sanya City of Hainan Province were collected from Jan 1, 2019 to Dec 31, 2019. Bacteria identification and drug sensitivity test were determined by bacterial identification and drug sensitivity analyzer, and supplemented by paper diffusion method. Results were determined according to the CLSI standards, and data were analyzed by using WHONET 5.6 software. RESULTS A total of 3853 clinical isolates were collected from Jan to Dec 2019, of which 858 strains were gram positive, accounting for 22.27%, and 2603 strains were gram negative, accounting for 67.56%. The average detection rate of methicillin-resistant strains in S. aureus（MRSA） and coagulase negative Staphylococcus（MRCNS）were 11.29% and 43.31%, respectively. The drug-resistance rates of MR strains to most of other antimicrobial agents were much higher than those of MS strains. No Staphylococcal strains were found resistant to vancomycin.The drug resistance rates of E. faecalis strains to most tested drugs were much lower than those of E. faecium; strains resistant to vancomycin were not found. The non-meningitis strains of S.pneumoniae had a drug resistance rate of 6.25% to penicillin. The drug resistance rates of A. baumannii strains to imipenem and meropenem were 29.21% and 34.92%, respectively. The drug resistance rates of P. aeruginosa strains to imipenem and meropenem were 1.57% and 1.83%, respectively. CONCLUSION The resistance of clinical isolates to common antimicrobial drugs is still high; especially strains in all bacteria genera of Enterobacteriaceae were carbapenems resistant. Therefore, clinical monitoring of bacterial resistance should be strengthened to prevent the further spread of drug-resistant bacteria.