Abstract: OBJECTIVE To analyze the features of postoperative incision infection, pathogens distribution, and its related risk factors in general surgery patients of the hospital from 2018-2019. METHODS A total of 2673 patients undergoing surgery in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from Jan 2018 to Dec 2019 were enrolled as the study subjects. Pathogens identification and drug resistance analysis were performed on specimens from patients with postoperative incision infection. The clinical data of patients were collected. The risk factors for incision infection were analyzed. RESULTS Among the 2673 patients in general surgery, there were 78 cases（2.92%） with postoperative incision infection, including 8 cases with type I incision infection, 29 cases with type II and 41 cases with type III. There were 84 strains of pathogens isolated from incision secretions, including 54 strains of gram-negative bacteria（64.29%）, 28 strains of gram-positive bacteria（33.33%） and 2 strains of fungi（2.38%）. The results of univariate analysis and multivariate Logistic regression analysis showed that age not younger than 65 years old, diabetes, hypoproteinemia, operation time not shorter than 3 h, emergency surgery, consecutive operation, type-III surgical incision, length of surgical incision not shorter than 10 cm, number of visitors not fewer than 3, no prophylactic usage of antibacterial agents, and hospitalization time not shorter than 10 d were independent risk factors for postoperative incision infection in general surgery（P＜0.05）. CONCLUSION The occurrence of surgical incision infection in general surgery patients is affected by patients’ conditions and operating room. And gram-negative bacteria are the main pathogenic bacteria. Effective intervention should be conducted based on the risk factors to reduce incidence of surgical incision infection.