Abstract: OBJECTIVE To detect carbapenem resistant genes of carbapenem-resistant Acinetobacter baumannii（CRAB） in infection patients in the hospital from 2018 to 2019. METHODS The specimens of patients in Hainan geriatric hospital during the period from Jan 2018 to Dec 2019 were retrospectively analyzed. The pathogens identification and drug sensitivity test were conducted by automatic microbiological analyzer. Two hundred CRAB strains isolated from the samples and were randomly detected by polymerase chain reaction assay to detect the resistant genes of OXA-51, OXA-58, OXA-23, OXA-24, IMP and VIM. RESULTS From 2018 to 2019, there were 12 121 strains of pathogens detected from the infected patients, mainly including Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumannii. From 2018 to 2019, CRAB detected in nosocomial infection patients accounted for 52.02%, carbapenem-resistant P. aeruginosa（CRPA） accounted for 22.93% and carbapenem-resistant Klebsiella pneumoniae（CRKP） accounted for 2.30%. CRAB was mainly detected from respiratory tract specimens, and mainly distributed in the intensive care unit, department of respiratory medicine and department of neurology. CRAB strains were highly resistant to ampicillin/sulbactam, furantoin and cefotetan, and more sensitive to ticecycline and polymyxin B. The detection rates of CRAB and CRPA strains in 2019 were significantly higher than those in 2018（P＜0.05）. The drug resistance rate of CRAB strains to ampicillin/sulbactam in 2019 was significantly lower than that in 2018（P＜0.05）, while that of CRAB strains to sulfamethoxazole/trimethoprene, amikacin, ceftazidime, tobramycin, and tegacycline in 2019 were significantly higher than that in 2018（P＜0.05）. The carbapenase resistant genes in 200 CRAB strains were mainly OXA-51 and OXA-23. CONCLUSION CRAB-infection patients from 2018 to 2019 were mainly distributed in the departments mainly including intensive care unit and department of respiratory medicine. The infection was mostly at respiratory tract and drug resistance genes were mainly OXA-51 and OXA-23. Clinically, routine drugs such as amikacin, ceftriaxone combined with tigecycline or polymyxin B can be applied for treatment.