Abstract: OBJECTIVE To explore the detection characteristics of drug-resistant Staphylococcus aureus and the expression changes of femA, mecC and PVL in a pediatric department of a tertiary hospital. METHODS Total of 149 strains of S. aureus isolated from clinical specimens of outpatients and inpatients from Jan 2018 to Mar 2020 of Binzhou Hospital of Traditional Chinese Medicine were divided into the methicillin-resistant S. aureus（MRSA） group and methicillin-sensitive S. aureus（MSSA） group. The source distribution of specimens detected S. aureus and drug resistance of the strains between the MRSA group and MSSA group were compared. The clinical data and prognosis between the two groups of children were compared. Polymerase chain reaction was used to detect drug resistance genes of fema, mecC and PVL. RESULTS Among 149 SA strains detected, 61 strains were detected from pus specimens, accounting for 40.94%, with the highest detection rate. The detection rate of MRSA was 35.57%（53/149）; the drug resistance rate to clindamycin in the MRSA group was significantly higher than that in the MSSA group（P＜0.05）. The drug resistance rates of S. aureus detected to tetracycline, teraconine and linezolid were all 0. The detection rate of femA gene in the MRSA group and the MSSA group were both 100.00%, while he mecC detection rate in both groups were 0, and there was no significant difference in PVL detection rate. CONCLUSION There are no significant differences in SA strains carrying drug resistance genes such as femA, mecC and PVL in this area between our study and domestic reports. The prognosis of children with MRSA infection is relatively poor, and continuous attention should be paid to provide complete data for infection prevention and control.