Abstract: OBJECTIVE To investigate the distribution and drug resistance of clinical isolates from bronchoalveolar lavage fluid（BALF） specimens in Gansu Province in 2019.METHODS The data of surveillance of drug resistance of bacteria isolated from BALF were collected from 74 hospitals of Gansu Province from Jan 2019 to Dec 2019. The drug susceptibility of the strains was determined by using disk diffusion method, minimum inhibitory concentration（（MIC）） method and E-test.RESULTS A total of 1 043 strains of bacteria that were isolated from BALF specimens were involved in the study in Gansu Province in 2019, 270（25.89%） of which were gram-positive bacteria, 773（74.11%） were gram-negative bacteria; the isolation rates of methicillin-resistant Staphylococcus aureus（MRSA） and methicillin-resistant coagulase-negative Staphylococcus（MRCNS） were relatively high. The drug resistance rates of MRSA strains were higher than those of methicillin-sensitive S.aureus（MSSA） strains. No vancomycin-, teicoplanin-or linezolid-resistant strains were found. The detection rate of extended-spectrum β-lactamases（ESBLs） was higher in Escherichia coli strains than in Klebsiella pneumoniae strains. The drug resistance rate of Acinetobacter baumannii strains to carbapenems was higher than that of Pseudomonas aeruginosa strains.CONCLUSION The gram-negative bacteria are dominant among the pathogens isolated from BALF specimens in Gansu Province, the drug resistance rates of the strains are high. The analysis of the drug resistance of the strains isolated from BALF may facilitate the reasonable use of antibiotics for the patients with different types of pulmonary infection so as to avoid the abuse of antibiotics.