Abstract: OBJECTIVE To understand the drug resistance of methicillin-resistant S.aureus（MRSA） and genotypes of Staphylococcus aureus cassette chromosome（SCCmec） so as to detect the genes of mecA and leukocidin.METHODS A total of 173 non-repetitive strains of S.aureus isolated from clinical samples were collected from a comprehensive Kiang Wu Hospital in Macao between Sep 2017 and Apr 2018. The drug susceptibility testing was performed for 16 types of commonly used antibiotics by Vitek-2, the phenotypes of MRSA were determined by using Cefoxitin disk method, and mecA,PVL and SCCmec geneⅠ-Ⅴ genotypes were detected by means of polymerase chain reaction（PCR）.RESULTS Among 173 strains of S.aureus, 51 were tested positive for MRSA phenotypes, accounting for 29.48%, 49 of which carried with mecA gene, and 20 carried with PVL gene. The incidence of MRSA infection was significantly higher among the patients aged no less than 55 years old（P＜0.05）. The result of drug susceptibility testing of 51 MRSA strains for 16 types of antibiotics showed that all of the strains were sensitive to quinupristin, linezolid, vancomycin and tigecycline, and the drug susceptibility rates to gentamicin, ciprofloxacin, levofloxacin, moxifloxacin, erythromycin, clindamycin, tetracycline and rifampicin ranged between 27.5% and 98%; there was no significant difference in the drug resistance among the MRSA strains with different SCCmec genotypes. 22 strains of SCCmecⅣ genotype were dominant among the 51 strains of MRSA, followed by 11 strains of SCCmecⅡ genotype, 9 strains of SCCmecⅤ genotype, 6 strains of SCCmecⅢ genotype and 3 strains of MRSA with unknown genotype.CONCLUSION The detection rates of MRSA and PVL gene are declined in Macao as compared with those in 2016, the SCCmecⅣ genotype is prevalent in Macao, the genotyping of SCCmec is not associated with the drug resistance in the MRSA strains, and the PVL gene is not associated with the drug resistance in MRSA or the genotyping of SCCmec.