Abstract: OBJECTIVE To explore the influence of high-fat diet on Helicobacter pylori infection-induced gastric mucosal injury and gastrointestinal hormones of mice.METHODS The SPF-level C57 BL/6 j mice were divided into the control group, the high-fat diet group, the Hp infection group and the high-fat diet + Hp infection group, with 15 mice in each group. The gastric mucosal lesions were observed by hematoxylin-eosin（HE） staining so as to obtain the scores of gastric mucosal lesions. The levels of gastrointestinal hormones in gastric tissues and plasma and inflammatory factors in gastric tissues were detected by enzyme-linked immunosorbent assay（ELISA）, and the expression levels of Wnt/β-catenin signal pathway-related proteins and genes were detected by means of Western blot and reverse transcription-polymerase chain reaction（RT-PCR）.RESULTS The result of HE showed that the scores of gastric mucosal lesions of the high-fat diet group and the Hp infection group were significantly higher than those of the control group（P＜0.05）; the score of gastric mucosal lesions of the high-fat diet + Hp infection group was significantly higher than that of the high-fat diet group and the Hp infection group（P＜0.05）. The levels of serotonin（5-HT）, gastrin（Gas） and motilin（MTL） in gastric tissues and plasma of the high-fat diet group were significantly higher than those of the control group（P＜0.05）, while the levels of somatostatin（SS） and ghrelin（Grelin） of the high-fat diet group were significantly lower than those of the control group（P＜0.05）. The levels of 5-HT, Gas and MTL in gastric tissues and plasma of the high-fat diet plus Hp infection group were significantly higher than those of the high-fat diet group and the Hp infection group（P＜0.05）; while the levels of SS and Grelin of the high-fat diet plus Hp infection group were significantly lower than those of the high-fat diet group and the Hp infection group（P＜0.05）. The levels of tumor necrosis factor-ɑ（TNF-ɑ）, interleukin（IL）-6 and IL-1β in gastric tissues of the high-fat diet group and the Hp infection group were significantly higher than those of the control group（P＜0.05）, while the IL-10 level of the high-fat diet group and the Hp infection group was significantly lower than that of the control group（P＜0.05）; the levels of TNF-ɑ, IL-6 and IL-1β in gastric tissues of the high-fat diet plus Hp infection group were significantly higher than those of the high-fat diet group and the Hp infection group（P＜0.05）, while the IL-10 level of the high-fat diet plus Hp infection group was lower than those of the high-fat diet group and the Hp infection group（P＜0.05）. The levels of Wnt3 a, β-catenin protein and mRNA in gastric tissues of the high-fat diet group and the Hp infection group were significantly higher than those of the control group（P＜0.05）; the levels of Wnt3 a, β-catenin protein and mRNA in gastric tissues of the high-fat diet plus Hp infection group were significantly higher than those of the high-fat diet group and the Hp infection group（P＜0.05）.CONCLUSION The high-fat diet may aggravate the gastric mucosal injury induced by Hp infection, inflammation response in gastric tissues and disorders of gastrointestinal hormones and also affect the activity of Wnt/β-catenin signaling pathway.