Abstract: OBJECTIVE To investigate the current status of implementation of specifications and related supplementary measures for cleaning and disinfection of duodenoscopes in China and find out the problems in cleaning and disinfection of the duodenoscopes.METHODS By means of convenient sampling, a questionnaire survey concerning configuration status of equipment, cleaning procedures, use and storage of disinfectants, allocation and training of personnel and implementation of four supplemental measures was conducted for 142 hospitals in which endoscopic retrograde cholangiopancreatography was performed.RESULTS The survey involving 142 hospitals covered all of the provincial administrative regions of China, the tertiary hospitals accounted for 95.77%, the number of duodenoscopes ranged between 1 and 5, the diagnosis and treatment volume was less than 500 cases in 86.62% of the hospitals, automatic cleaning and disinfection machines were used for reprocessing in 80.99% of the hospitals. The rate of completing all the key points in WS 507-2016 "Technical Specifications for Cleaning and Disinfection of Flexible Endoscopes" was 72.54%. The hospitals with insufficient cleaning of special structures accounted for 2.36%～70.08%, the hospitals with insufficient related consumables accounted for 14.79%, the hospitals with unreasonable test of concentration of disinfectants accounted for 6.34%, the hospitals with unreasonable storage of disinfectants accounted for 7.74%, and the hospitals with unreasonable drying accounted for 7.75%. The rate of implementation of the supplemental disinfection measures（EtO sterilization/liquid chemical sterilization/repeated HLD） ranged between 2.82% and 24.65%. The personnel who engaged in cleaning of endoscopes were lack of experience and had low education level.CONCLUSION The cleaning and disinfection facilities are equipped more completed for the duodenoscopes and better used than for the common endoscopes, but there are still insufficient cognition and implementation of the key points for cleaning and disinfection. It is necessary to strengthen the qualification and training of the personnel who are engaged in the cleaning and disinfection of duodenoscopes, and the rate of implementation of supplemental measures needs to be raised in China.