Abstract: OBJECTIVE To improve the timeliness of orders by using the electronic pre-warning and barrier technology of isolated doctor’s orders, and evaluate its impact on multidrug-resistant organisms（MDROs） nosocomial infection.METHODS The carbapenem-resistant Enterobacteriaceae（CRE）, carbapenem-resistant Acinetobacter baumannii（CRAB）, carbapenem-resistant Pseudomonas aeruginosa（CRPA）, methicillin-resistant Staphylococcus aureus（MRSA）, vancomycin-resistant Enterococci（VRE） and extended-spectrum β-lactamases（ESBLs）-producing Enterobacter strains that were isolated from the hospitalized patients of Shenzhen Hospital of Guangzhou University of Traditional Chinese Medicine were enrolled in the study. The time period from Jan 2019 to Jul 2019 was assigned as the pre-intervention group, while the time period from Aug 2019 to Apr 2020 was assigned as the intervention group. The critical value management was carried out for the drug-resistant strains, the information pre-warning and technology barrier of isolation orders was achieved in electronic medical order, nursing medical order and Picture Archiving and Communication Systems（PACS）. The time periods of the doctors’ and nurses’ executing the isolation orders were observed before and after the intervention measures were taken, and the intervention effects on MDROs nosocomial infection were observed.RESULTS After the implementation of pre-warning and barrier measures for isolation orders, the time for doctors issuing isolation orders was shortened from 1323 minutes to 238 minutes（P＜0.05）.The time for nurses to execute isolation orders was shortened from 31 minutes to 13 minutes（P＜0.05）. The infection and infection case rate of CRE all decreased from 0.04% to 0.01%,the infection rate of CRAB decreased from 0.18% to 0.05%, the infection case rate of CRAB decreased from 0.21% to 0.05%,the infection case rate of ESBLs decreased from 0.33% to 0.23%,the differences were significant（P＜0.05）,there was a positive correlation of the nosocomial infection rate and infection case rate of CRE and CRAB with the shortening of doctor’s isolation order time（P＜0.05）.CONCLUSION The electronic pre-warning and technical barrier of MDROs help to improve timeliness of doctors and nurses to implement isolated doctor’s orders, and it would play a positive role in the prevention of nosocomial infection caused by CRE, CRAB.