Abstract: OBJECTIVE To explore the expression of long non-coding RNA CRNDE and miR-182 in women with premature rupture of membranes and intrauterine infection and their relationship with neonatal brain damage. METHODS A total of 113 cases of PROM and intrauterine infection women who gave birth in the neonatal intensive care unit of Xinxiang Central Hospital from Mar 2018 to May 2020 were selected as the research subjects and divided into 42 cases of brain injury group and 71 cases pf the non-brain injury group according to whether the newborns delivered by the included mothers had brain damage. The peripheral blood samples was collected from all parturients on the day of delivery, and RNA was extracted from peripheral blood. Real-time fluorescent quantitative polymerase chain amplification reaction was used to detect the expression of LncRNA, CRNDE and miR-182. The relationship between LncRNA CRNDE and miR-182 levels and the severity of neonatal brain injury and brain injury was analyzed, and ROC was used to analyze the predictive value. RESULTS The levels of LncRNA CRNDE and miR-182 in the brain injury group were significantly higher than those in the non-brain injury group（P＞0.05）; The levels of LncRNA CRNDE and miR-182 in the severe brain injury group were significantly higher than those in the moderate brain injury group and the mild brain injury group. The levels of LncRNA CRNDE and miR-182 in the moderate brain injury group were significantly higher than those in the mild brain injury group（P＜0.05）; ROC analysis results showed that the area under the curve of LncRNA CRNDE and miR-182 in maternal peripheral blood for the prediction of neonatal brain injury on the postpartum day was 0.755 and 0.742, respectively, which were significantly different（P＜0.05）.CONCLUSION The levels of long-chain non-coding RNA CRNDE and miR-182 in postpartum peripheral blood had certain clinical value for predicting the occurrence of neonatal brain injury and assessing its severity.