Abstract: OBJECTIVE To analyze the serum levels of soluble leukocyte differentiation antigen 14 subtype（sCD14-ST, presepsin）, endotoxin, interleukin（IL）-6, procalcitonin（PCT）, and their prognostic values in patients with sepsis in the intensive care unit（ICU）. METHODS A total of 106 patients with sepsis admitted to ICU of Haikou People's Hospital from Jan 2018 to Jan 2020 were selected as the research subjects. According to the severity of illness, they were divided into 44 cases of the sepsis group, 42 cases of the severe sepsis group, and 20 cases of the septic shock group. And according to the prognosis, they were further divided into 88 cases of the survival group and 18 case of the death group. The levels of white blood cell（WBC）, platelet count（PLT） in the peripheral blood and the serum levels C-reactive protein（CRP）, lactic acid（LAC）, Presepsin, endotoxin, IL-6 and PCT were compared among each group within 24 hours after ICU treatment. RESULTS With the aggravation of the disease, the level of PLT in peripheral blood decreased gradually, and the level of other laboratory indexes increased significantly（P＜0.05）. The level of PLT was negatively correlated with the severity of disease, while the levels of other laboratory indexes were positive correlated with the severity of disease（P＜0.05）. The serum levels of presepsin, endotoxin, IL-6 and PCT of the patients in the death group were higher than those of the patients in the survival group and the differences were significant（P＜0.05）. The areas under receiver operating characteristic curve of the serum levels of presepsin, endotoxin and IL-6 in prediction of the death of patients with sepsis were statistically significant（P＜0.05）. CONCLUSION The serum levels of Presepsin, endotoxin, IL-6 and PCT in the early stage of the patients with sepsis in ICU were correlated with the severity of disease, which can be used to assist in predicting the risk of death. Clinicians should consider using these new markers to improve the efficiency of clinical evaluation.