Abstract: OBJECTIVE To investigate the pathogenic bacteria biofilm formation and prognosis factors in patients with catheter-related fungal bloodstream infection. METHODS A total of 45 patients with catheter-related fungal bloodstream infection admitted to the 904 th Hospital of Joint Logistics Support Force of PLA from Apr 2018 to Aug 2020 were selected as the study subjects. Strain identification and drug sensitivity analysis were conducted. The strains were cultured, and biofilm formation was observed under electron microscope. The treatment status of patients and mortality rate within 30 days were recorded. RESULTS A total of 45 strains of fungi were isolated from 45 patients with catheter-related fungal bloodstream infection, and 13 fungal biofilms were formed from clinically isolated fungal strains, with a positive rate of 28.89%. The resistance rate of strains with biofilm formation to itraconazole, fluconazole and voriconazole-resistant strains and the proportion of intermediary strains were significantly higher than those without biofilms（P＜0.05）. Of the 45 patients with fungal bloodstream infection, 32 patients accounting for 71.11% received empirical antifungal treatment, 18 patients accounting for 56.25% received sensitive drug treatment, and 14 patients accounting for 43.75% were given non-sensitive drugs. The 30-day mortality rate in this study was 14 patients, accounting for 31.11%. Univariable analysis showed that the use of sensitive antibiotics was related to the 30-day mortality rate; Logistic regression analysis showed that the use of sensitive antibiotics was a protective factor of the 30-day mortality rate（P＜0.05）. CONCLUSION The formation of Fungal biofilm was related to drug resistance, and drug susceptibility was an influencing factor for the prognostic of patients with catheter-related fungal bloodstream infection.