Abstract: OBJECTIVE To explore the expressions of thymic stromal lymphopoietin（TSLP）, interleukin-33（IL-33） and their receptors in epithelial cells of chronic rhinosinusitis（CRS） patients complicated with fungal infection and analyze the significance. METHODS A total of 126 patients with CRS who were treated in Qingyang People's Hospital of Gansu Province from Jun 2019 to Jun 2020 were recruited as the study subjects and divided into the fungal rhinosinusitis（FRS） group with 60 cases and the CRS group with 63 cases. The epithelial cells were collected from nasal mucosa of the patients within 24 hours after confirmed diagnosis, cDNA was acquired by RNA extraction and reverse transcription, the expression levels of TSLP, IL-33 and their receptors were detected by real-time fluorescent quantitative PCR, and the expression levels of TSLP, IL-33 and their receptors were compared between the two groups. RESULTS The expression levels of TSLP, thymic stromal lymphopoietin receptor（TSLPR） and interleukin-7 receptor alpha chain（IL-7Rα） of the FRS group were higher than those of the CRS group（P＜0.05）. The expression levels of IL-33, transmembrane growth-stimulating protein（ST2L）, soluble growth-stimulating protein（sST2） of the FRS group were higher than those of the CRS group（P＜0.05）. CONCLUSION The expression levels of TSLP,IL-33 and their receptors of the FRS patients were higher than those of the CRS patients. The infection can be controlled by reducing the expression levels of TSLP, IL-33 and their receptors, which may provide guidance for clinical treatment of FRS.