Abstract: OBJECTIVE To explore the establishment of infection control supervision group and observe the effect of supervision on prevention of urinary catheter-associated urinary tract infection（CAUTI）. METHODS A total of 1 576 patients who were hospitalized and treated with urinary catheter indwelling in intensive care unit（ICU） of the First Affiliated Hospital of Air Force Medical University from Jan 2015 to Jun 2016 were chosen as the control group, and 2 051 urinary catheter indwelling patients who were hospitalized in the ICU from Jul 2016 to Jan 2018 were assigned as the intervention group, the infection control supervision was carried out. The supervision period of the intervention group was Jul 2016, the intervention period ranged between Aug 2016 and Jan 2018. The incidence of CAUTI was compared between the control group and the intervention group before and after intervention, and the compliance rates of prevention measures for CAUTI and hand hygiene of the intervention group were compared between the supervision period and the intervention period. RESULTS In terms of the prevention measures for CAUTI, the compliance rate of urine bag location of the intervention group was 87.26% during the supervision period, 97.51% during the intervention period, and there was significant difference（P＜0.05）. The compliance rates of other prevention measures were more than 97% during the intervention period. The compliance rate of hand hygiene before and after contact with urinary catheter system was 40.54% during the supervision period, 84.70% during the intervention period, and there was significant difference（P＜0.05）. The incidence of CAUTI was 4.31% in the control group, 1.75% in the intervention group, and there was significant difference（P＜0.05）. CONCLUSION The establishment of infection control supervision group and supervision may effectively reduce the incidence of CAUTI in ICU patients of neurosurgery department.