Abstract: OBJECTIVE To investigate the drug resistance and molecular characteristics of pathogens isolated from foot secretions of patients with diabetic foot infection（DFI）. METHODS The foot secretions were collected from 135 patients with DFI who were treated in endocrinology department of Hanchuan People's Hospital form Aug 2019 to Aug 2020, the drug susceptibility testing was carried out for the pathogens by using Phoenix 100 automatic bacterial identification/drug susceptibility test board with supporting drug susceptibility system. The DNA of the pathogens was extracted, and molecular typing was performed by multilocus sequence typing（MLST） or enterobacterial repetitive intergenic consensus-polymerase chain reaction（ERIC-PCR）. RESULTS Totally 228 strains of pathogens were isolated from foot secretions of the 135 patients with DFI, 88（38.60%） of which were gram-positive bacteria, 136（59.65%） were gram-negative bacteria; Staphylococcus aureus and Enterococcus faecalis were the predominant species of the gram-positive bacteria; Proteus and Escherichia coli were dominant among the gram-negative bacteria. The gram-positive bacteria were mainly sensitive to vancomycin, linezolid, quinupristin-dalfopristin and teicoplanin; the gram-negative bacteria were mainly sensitive to piperacillin-tazobactam, imipenem and meropenem. The result of ERIC-PCR typing showed that among the 36 strains of Proteus, there were 11 strains of type Ⅰ, 6 strains of type Ⅱ, 8 strains of type Ⅲ and 11 strains of type Ⅳ. MLST was carried out successfully for 22 of 30 E.coli isolates, the order of ST from high to low was as the following: ST94, ST6010, ST226, ST6005,ST69 and ST6006. MLST was carried out successfully for 20 of 26 S.aureus isolates, and the order of ST from high to low was as follow: ST59, ST630, ST88, ST188 and ST6. CONCLUSION Type Ⅰ and Ⅳ Proteus, ST94 and ST6010 E.coli, and ST59 and ST630 S.aureus are the common species of pathogens isolated from the foot secretions of the patients with DFI. It is necessary for the hospital to pay attention to the drug resistance patterns.