Abstract: OBJECTIVE To explore the distribution of pathogens isolated from patients with diabetic foot and observe the clinical intervention effect of silver ion dressing. METHODS The patients with diabetic foot who were treated in endocrinology department of Dazhou Central Hospital from Jan 2020 to Dec 2020 were enrolled in the study, the medical data were collected from the patients, totally 68 diabetic foot patients who were tested positive for microorganisms were randomly divided into the control group and the intervention group, with 34 cases in each group. The secretion specimens were collected from the surfaces of wounds of diabetic foot, the pathogens were isolated and identified, the patients were treated with silver ion dressing for intervention therapy, and the clinical curative effect was evaluated based on specific test indexes. RESULTS All of the 68 diabetic foot patients were tested positive for culture of pathogens in wound secretions, totally 98 strains of pathogens were isolated, 59（60.20%） of which were gram-negative bacteria, 36（36.73%） were gram-positive bacteria, and 3（3.06%） were fungi. The positive rate of pathogens in wound secretions declined with the extension of intervention time, which declined most remarkably on day 20, and there was significant difference（P＜0.05）. The total effective rate of the intervention group was 94.12% after 20-day intervention, the control group 61.76%, and there was significant difference（P＜0.05）. CONCLUSION The gram-negative bacteria are dominant among the pathogens isolated from the patients with diabetic foot, the silver ion dressing can effectively improve bacterial infection and wound healing, and it has great significance in clinical treatment of diabetic foot ulcers（DFU） infection.