Abstract: OBJECTIVE To investigate the prevalence of nosocomial infection among hospitalized patients and understand the current status of control of nosocomial infection so as to improve the prevention strategies and provide guidance for control of infection. METHODS The patients who were hospitalized in PLA Strategic Support Force Medical Center form Jan 2021 to Dec 2021 were recruited as the study subjects. The wards of infection, infection sites, susceptible factors, urinary catheter-related infection and pathogens causing nosocomial infection were analyzed by means of Xinglin hospital infection real-time monitoring system software and electronic medical records management platform. RESULTS A total of 22 224 patients were hospitalized in the hospital in 2021, the infection rate was 2.05%, the case-time infection rate was 2.24%, the missing report rate was 14.25%, the missing case-time reporting rate was 13.28%, the utilization rate of urinary catheter was 12.37%, the incidence of urinary catheter-related urinary tract infection was 2.58‰. Among the patients with nosocomial infection, 46.68% had lower respiratory tract infection, 17.51% had urinary catheter-related infection. Among the susceptible factors for the nosocomial infection, the advanced age accounted for 29.09%, the urinary tract catheterization accounted for 9.13%, the central venous catheterization accounted for 7.43%, the endotracheal intonation accounted for 4.46%, and the all of the susceptible factors that were associated with the catheterization accounted for 26.54% in total. There was no significant difference in the distribution among the 15 species of pathogens causing nosocomial infection. CONCLUSION The control of nosocomial infection should focus on the prevention of lower respiratory tract infection, urinary catheter-related urinary tract infection and multidrug-resistant organisms infections. It is necessary for the hospital to pay great attention to the incidence and control of nosocomial infection so as to reduce the incidence of nosocomial infection.