Abstract: OBJECTIVE To investigate the factors influencing norovirus (NoV) infection in acute gastroenteritis and its genetic characteristics. METHODS A total of 102 patients with NoV-infected acute gastroenteritis in Wuhan Wuchang Hospital were selected to be included in the case group between Jan. 2019 and Jun. 2021, and aother 102 healthy people without diarrhea or vomiting within 2 weeks were selected as the control group according to matched gender, age and occupation. The diet and personal hygiene between the two groups were compared. The influencing factors of NoV infection were analyzed by Logistic regression analysis, the dominant genotypes of NoV infection were detected, and the genetic characteristics were analyzed. RESULTS The proportions of patients with mixed usage of chopping board for raw and cooked food, frequent consumption of seafood-based foods, habit of not washing hand before eating and after toilet, eating at informal restaurants/mobile stalls, and exposure to vomiting or diarrhea people in the case group were higher than those in the control group (P<0.05). The mixed usage of chopping board for raw and cooked food, frequent consumption of seafood-based foods, habit of not washing hand before eating and after toilet, eating at informal restaurants/mobile stalls and exposure to vomiting or diarrhea people were influencing factors of NoV-infected acute gastroenteritis (P<0.05). In terms of amplification and typing of the RdRp region against ORF1 in 102 NoV-positive specimens, 71 cases were successfully typed, mainly on GII.Pe and GII.P12 (P<0.05). In terms of amplification and typing of Capsid region against ORF2, 66 cases were successfully typed, mainly on GII.4 Sydney2012 and GII.3 (P<0.05). There were 54 cases with successful typing for RdRp and Capsid fragments, mainly on GII.Pe-GII.4 Sydney2012 and GII.P12-GII.3 (P<0.05). CONCLUSION Poor diet and living habits were important causes of NoV virus infection. The prevalent strains were almost all recombinant strains, GII.Pe-GII.4 Sydney 2012 and GII.P12-GII.3 were the main recombinant strains, with simultaneously appeared recombinant strains of GII.Pe-GII.3 and GII.P16-GII.2.