Abstract: OBJECTIVE To analyze the drug resistance genes and characteristics of virulence factors of carbapenem-resistant Pseudomonas aeruginosa (PA). METHODS A total of 764 stains of PA bacteria isolated from focal secretions of patients with post-surgical infection in Shengjing Hospital Affiliated to China Medical University between Mar. 2018 and Mar. 2021 were collected for strain identification and drug sensitivity analysis. DNA of bacterial strains were extracted, and drug resistance genes and virulence factors were detected. RESULTS Totally 94 strains of CRPA were detected in 764 strains of PA, with a detection rate of 12.30%. The resistance rates of 94 CRPA strains to commonly used antibacterial drugs ranged from 55.32% to 98.94%. Drug resistance genes, including bla_GES, bla_IMP and bla_VIM were detected in 94 CRPA strains, with detection rates of 11.70%, 10.64% and 9.57%, respectively. Virulence factors, including exoU, exoS, popB, LasI, Hcp2 and LasR were detected in 94 CRPA strains, with detection rates of 65.96%, 46.81%, 100.00%, 86.17%, 76.60% and 89.36%, respectively. CONCLUSION The CRPA strains were highly resistant to all commonly used antibacterial drugs, but were relatively sensitive to amikacin, levofloxacin, gentamicin and ceftazidime. Drug resistance genes containing bla_GES, bla_IMP, and bla_VIM, and virulence factors carrying exoU, exoS, popB, LasI, Hcp2 and LasR were detected.