Abstract: OBJECTIVE To analyze the factors influencing in-hospital methicillin-resistant Staphylococcus aureus (MRSA) infection in burn patients and its drug resistance and virulence genes. METHODS Ninety-five burn patients with hospital-acquired MRSA infection who were admitted to Daqing Oilfield General Hospital from May 2019 to May 2021 were selected as the study subjects and set as the study group, and 80 burn patients with hospital-acquired methicillin-sensitive Staphylococcus aureus (MSSA) infection during the same period were selected as the control group. The data of both groups were collected to summarize the influencing factors of MRSA infection in burn patients, and the drug resistance of MRSA and MSSA was analyzed. The status of drug resistance genes and virulence genes were detected by polymerase chain reaction (PCR) method. RESULTS The detection rate of MRSA infection in burn patients was 54.29%. Time of antimicrobial administration, invasive procedures, anemia, and hypoproteinemia were the influencing factors of MRSA infection in burn patients (P<0.05). The resistance rates of MRSA to penicillin, oxacillin, tetracycline, gentamicin, ciprofloxacin, levofloxacin, rifampicin, clindamycin, and erythromycin were higher than 70.00%. The virulence genes carried by MRSA were sea, hla and pvl, with carriage rates of 100.0%, 100.0% and 60.00%, respectively. The drug resistance genes carried by MRSA were mecA, aac(6')/aph(2''), ermA/B/C, tet/M, aph(3')-Ⅲ and qacA/B, with carriage rates of 100.0%, 100.0%, 100.0%, 100.0%, 96.84% and 86.32%, respectively. CONCLUSION The duration of antimicrobial medication, invasive procedures, anemia, and hypoproteinemia were the influencing factors of MRSA infection in burn patients. MRSA carried drug resistance and virulence genes, suggesting that MRSA had strong host tissues damage characteristics, and clinical antibacterial drugs should be selected reasonably according to the condition and drug susceptibility test to reduce MRSA infection.