Abstract: OBJECTIVE To analyze the relationship between expressions of latent membrane protein-1 (LMP-1), p53 and Ki67 in peripheral blood and malignant progression of tumor in patients with nasopharyngeal carcinoma complicated with Epstern-Barr (EB) virus infection. METHODS Totally 60 patients with nasopharyngeal carcinoma complicated with EB virus infection who were treated in Henan Provincial People' s Hospital between May 2018 and May 2021 were selected and included in the infection group, and 42 patients with nasopharyngeal carcinoma without EB virus infection were included in non-infection group. The expressions of LMP-1, p53 and Ki67 in peripheral blood at the time of admission were compared between the two groups, and the relationship between expressions of LMP-1, p53 and Ki67 in peripheral blood and nasopharyngeal carcinoma with EB virus infection was analyzed by Logistic regression equation. The relationship between expressions of peripheral blood LMP-1, p53 and Ki67 and clinicopathological characteristics of patients with nasopharyngeal carcinoma without EB virus infection was analyzed. According to the presence or absence of lymph node metastasis and distant metastasis, the infection group was divided into different subgroups, and the expressions of peripheral blood LMP-1, p53 and Ki67 were compared among different subgroups. RESULTS The positive rates of LMP-1, p53 and Ki67 in peripheral blood were higher in infection group than those in non-infection group (P<0.05). The positive expressions of peripheral blood LMP-1, p53 and Ki67 were the risk factors for EB virus infection in patients with nasopharyngeal carcinoma (P<0.05). There were statistical differences in the expressions of peripheral blood LMP-1, p53 and Ki67 among patients with nasopharyngeal carcinoma complicated with EB virus infection of different N stages (P<0.05), and the higher the N stage, the higher the positive expression rates of peripheral blood LMP-1, p53 and Ki67 (P<0.05). The positive rates of LMP-1, p53 and Ki67 expression in peripheral blood of patients with distant metastatic nasopharyngeal carcinoma combined with EB virus infection were higher than those of patients without distant metastasis (P<0.05). CONCLUSION The positive rates of LMP-1, p53 and Ki67 expression in peripheral blood were higher among patients with nasopharyngeal carcinoma complicated with EB virus infection and were the risk factors for EB virus infection in patients with nasopharyngeal carcinoma, and were closely related to the N staging and distant metastasis, which might be involved in the process of tumor malignant progression.