Abstract: OBJECTIVE To understand the drug resistance characteristics of clinical isolates of carbapenem-resistant Enterobacteriaceae (CRE), and to detect and analyze the carriage of disinfectant-resistant genes. METHODS A total of 210 non-repeating strains were obtained from various specimens including sputum, blood, pleural and ascites fluid from inpatients send to the First Hospital of Lanzhou University from Jan. to Dec. 2021. Strain identification and drug sensitivity test were carried out. Polymerase chain reaction (PCR) method was used to detect the carrying of disinfectant-resistant genes. RESULTS The 210 CRE strains were mainly Klebsiella pneumoniae and Enterobacter cloacae, accounting for 64.29% (135/210) and 22.86% (48/210), respectively. The distribution of units was mainly in intensive care unit, accounting for 46.67% (98/210). The sources of specimens were mainly sputum of 49.52% (104/210), blood of 12.86% (27/210), and secretions of 9.52% (20/210) respectively. Among the 210 CRE strains, the antimicrobial drug with the lowest resistance rate was amikacin (49.52%). The resistance rates to gentamicin, tetracycline, levofloxacin, compound sulfamethoxazole, aztreonam and ertapenern were 67.62%, 72.38%, 83.33%, 75.71%, 84.76% and 98.10%, respectively, and the resistance rates to the rest of antibiotics were as high as 100.00%. The qacA, qacC and qacJ genes were not detected in the 210 CRE isolates. The positive rates of qacB, qacG, qacH, qacE, qacE△1 and qacE△1-SUL1 genes were 1.43%(3/210), 0.95%(2/210), 1.90%(4/210), 66.67%(140/210), 80.95%(170/210) and 91.43%(192/210), respectively. CONCLUSION The CRE strains were generally resistant to commonly used antibiotics, therefore, the monitoring of resistance of CRE strains should be strengthened, and antibiotics should be rationally selected according to the results of drug sensitivity to reduce the generation of resistant strains. The carriage rate of disinfectant-resistance genes in clinical isolates of CRE was high and dominated by qac E, qacE△1and qac.E△1-SUL1 The surveillance of disinfectant-resistant strains should be further strengthened, and the use of disinfectants should be reasonably adjusted according to the situation of disinfectant resistance to prevent the occurrence of nosocomial infection.