Abstract: OBJECTIVE To observe the distribution of pathogens isolated from the diabetes mellitus patients with urinary tract infection and analyze the influencing factors. METHODS A total of 342 patients with diabetes mellitus who were treated in Zhoukou Central Hospital from Sep 2019 to Sep 2022 were enrolled in the study and were divided into the no urinary tract infection group with 309 cases and the urinary tract infection group with 33 cases according to the status of urinary tract infection. The clinical data were retrospectively collected from the patients. The distribution of pathogens isolated from the diabetes mellitus patients complicated with urinary tract infection was observed. Univariate analysis and multivariate logistic regression analysis were performed for the influencing factors for the urinary tract infection in the diabetes mellitus. RESULTS Of the 342 diabetes mellitus, 33 had urinary tract infection, with the infection rate 9.65%. Totally 40 strains of pathogens were isolated from the 33 diabetes mellitus patients complicated with urinary tract infection, 22 (55.00%) of which were gram-negative bacteria, 15 (37.50%) were gram-positive bacteria, and 3 (7.50%) were fungi. Multivariate logistic analysis showed that female patient, rise of serum amyloid A(SAA) and urinary catheter indwelling were the influencing factors for the urinary tract infection in the diabetes mellitus patients (P＜0.05). CONCLUSION The incidence of urinary tract infection is high among the patients with diabetes mellitus. The gram-negative bacteria are dominant among the pathogens causing the infection. The incidence of urinary tract infection in the patients with diabetes mellitus is closely associated with the female patient, serum SAA level and urinary catheter indwelling.