肺炎支原体感染临床特征及TGF-β1/Smads通路变化

Changes of TGF-β1/Smads pathways and clinical characteristics of patients with Mycoplasma pneumoniae infection

    摘要
  • 摘要: 目的 探讨肺炎支原体感染患者临床特征与TGF-β1/Smads信号通路在免疫反应中的作用。方法 选取2021年6月-2022年5月在内蒙古医科大学附属医院就诊的120例肺炎支原体感染患者为研究对象,其中规范性地应用大环内酯类抗菌药物治疗≥7 d的60例患者为难治性肺炎支原体肺炎(RMPP)组,其余60例患者为支原体肺炎(MPP)组;按照随机数字表法从2022年在医院进行查体的健康人群中选择60名为对照组。对两组患者临床症状与生命质量进行评估,测定三组TGF-β1/Smads信号通路相关蛋白与mRNA水平、炎症因子水平以及T淋巴细胞亚群水平。结果 RMPP组临床症状发生率大多高于MPP组(P＜0.05);对照组生命质量评分高于MPP组,MPP组高于RMPP组(P＜0.05);MPP组和RMPP组血清C-反应蛋白(CRP)、干扰素γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-4、IL-6、IL-10水平均要高于对照组,IL-1β低于对照组(P＜0.05);RMPP组CD3+、CD4+、CD8+水平最低,低于对照组与MPP组,CD4+/CD8+对照组最低,RMPP组最高(P＜0.05);MPP组和RMPP组TGF-β1、Smad3蛋白与mRNA表达水平高于对照组,Smad7蛋白与mRNA表达水平低于对照组(P＜0.05)。结论 MP感染与患者体内TGF-β1/Smads信号通路有关,TGF-β1/Smads信号通路可介导支原体肺炎患者的免疫反应。

     

    Abstract: OBJECTIVE To explore the clinical characteristics of the patients with Mycoplasma pneumoniae infection and observe the effect of TGF-β1/Smads signaling pathways on immune response. METHODS A total of 120 patients with M.pneumoniae infection who were treated in the Affiliated Hospital of Inner Mongolia Medical University from Jun 2021 to May 2022 were recruited as the research subjects, 60 of whom were treated with macrolides for no less than 7 days and were assigned as the refractory M.pneumoniae pneumonia (RMPP) group, and the rest of 60 patients were assigned as the MPP group; 60 healthy people who received physical examination in 2022 were chosen as the control group. The clinical symptoms and quality of life (QOL) were assessed, and the levels of TGF-β1/Smads signaling pathways proteins, mRNA, inflammatory factors and T lymphocyte subsets were detected for the three groups. RESULTS The incidence of clinical symptoms of the RMPP group was higher than that of the MPP group (P＜0.05). The score of QOL of the control group was higher than that of the MPP group, and the score of QOL of the MPP group was higher than that of the RMPP group(P＜0.05). The levels of serum C-reactive protein (CRP), interferon-γ(IFN-γ), tumor necrosis factor-α(TNF-α), interleukin(IL)-4, IL-6 and IL-10 of the MPP group and the RMPP group were higher than those of the control group, while the IL-1β level of the MPP group and the RMPP group was lower than that of the control group(P＜0.05). The levels of CD3+, CD4+ and CD8+ of the RMPP group were lower than those of the control group and the MPP group; the CD4+/CD8+ level was the lowest in the control group, the highest in the RMPP group(P＜0.05). The expression levels of TGF-β1, Smad3 proteins and mRNA of the MPP group and the RMPP group were higher than those of the control group, while the expression levels of Smad7 and mRNA of the MPP group and the RMPP group were lower than those of the control group(P＜0.05). CONCLUSION The M.pneumoniae infection is associated with the TGF-β1/Smads signaling pathways. The TGF-β1/Smads signaling pathways can mediate the immune response of the patients with Mycoplasma pneumonia.

     

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