Abstract: OBJECTIVE To investigate the characteristics of pathogenic bacteria in patients with diabetic foot infection complicated with multidrug resistant bacteria, to construct a risk factor model and analyze its intervention measures. METHODS The clinical data of 219 patients with diabetic foot infection admitted to Liaocheng Hospital of Traditional Chinese Medicine from Jan 2019 to Dec 2022 were retrospectively enrolled as the research subjects. According to the types of infection, the patients were divided into the multidrug resistant bacteria group (n=98 cases) and non-multidrug resistant bacteria group (n=121 cases). The data of patients with diabetic foot infection were collected and the characteristics of pathogenic bacteria and multidrug resistant bacteria were analyzed. The drug resistance of the main multidrug resistant bacteria was analyzed. The multivariate logistic regression analysis was used to analyze the risk factors for the occurrence of multidrug resistant bacteria in patients with diabetic foot infection, and the risk factor model was established. The receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of the risk factor model for multidrug resistant bacteria in patients with diabetic foot infection. RESULTS Among the 219 patients with diabetic foot infection, 98 cases were complicated with multidrug resistant bacteria infection, from which 102 strains of multidrug resistant bacteria were detected, mainly Escherichia coli and Staphylococcus aureus. Older age, long duration of diabetic complications (>2 years), repeated infection, combined use of antibiotics (>2 kinds), long-term use of antibiotics, complicated with osteomyelitis, complicated with nerve ischemic wound, and more than two hospitalizations per year were independent risk factors for multidrug resistant bacteria in patients with diabetic foot infection (P＜0.05). Regression model was constructed as follows: Logit (P) =-10.497+ older age ×0.114+ duration of diabetic complications (>2 years) ×0.127+ recurrent infection ×1.021+ combined use of antibiotics (>2 kinds) ×1.088+ long-term use of antibiotics ×1.242+ combined osteomyelitis ×0.973+ combined nerve ischemic wound ×1.197 + hospitalizations for the same site occurred more than twice per year×1.075. The AUC of probability Logit (P) was 0.804, the sensitivity was 74.49%, and the specificity was 75.21%. CONCLUSION E. coli and S. aureus are the main pathogens causing multi-drug resistant bacteria in patients with diabetic foot infection. Many factors such as old age and long course of diabetic complications are closely related to multidrug-resistant bacteria in patients with diabetic foot infection.