Abstract: OBJECTIVE To explore the screening methods of high-risk human papillomavirus (HR-HPV) infection among the patients with cervical lesions and observe their application. METHODS A total of 1 405 patients with cervical lesions who were treated in obstetrics and gynecology department of the First Medical Center of Chinese PLA General Hospital from Jun 2018 to Dec 2020 were recruited as the research subject. The distribution of the patients with HR-HPV infection among the cervical lesion patients, single type, mixed types of HR-HPV infections and DNA copies were observed. The effectiveness of the screening strategies was explored. RESULTS Among the enrolled patients with cervical lesions, 613 had inflammation, 461 had low-grade squamous intraepithelial lesion (LSIL), 311 had high-grade squamous intraepithelial lesion (HSIL), and 20 had cervical cancer (CC). The HR-HPV 82, 31, 16, 68, 39, 33, 35 and 18 were the genotypes that were most likely to lead to cervical lesions. There was slight difference in the HR-HPV genotype between the CC patients and the precancerous lesion patients: HPV16 and HPV18 genotypes for the CC group; HPV16, 58, 52, 51, 31, 58 and 66 genotypes for the precancerous lesion group. Among the patients with single genotype of HR-HPV infection, the occurrence of cervical lesions showed closer association with the HPV31, 16, 52, 58, 56 and 18 genotypes. The risk of cervical lesions was increased when the DNA copies were no less than 10E4. Among the patients with mixed HR-HPV infections, the percentage of the patients with HSIL was higher in the mixed infection group with any two subtypes of HPV16 and 18 than in the mixed infection group without any two subtypes of HPV16 and 18(P＜0.05). The positive rate of single pathological examination for screening of HR-HPV infection and pathological HSIL+ was fairly similar to single TCT, the combination of the two screening methods did not increase the sensitivity of positive pathological detection, but the combination with DNA copy increased the sensitivity of screening. CONCLUSION The genotyping of HR-HPV infection combined with DNA copies is effective for screening of cervical lesions. It is necessary to attach great importance to the association between the genotypes of HR-HPV infections and the lesions so as to provide clinical recommendations.