Abstract: OBJECTIVE To understand the drug resistance rates of pathogens isolated from patients of infectious diseases department so as to provide guidance for reasonable clinical use of antibiotics. METHODS All of the surveillance data were extracted from the pathogens isolated from the infectious diseases departments of the members of China Antimicrobial Resistance Surveillance System (CARSS) from Oct 2020 to Sep 2021. The repetitive strains were eliminated based on the principle that the first strain of the same species from the same patient was eliminated, and the data were analyzed by using WHONET 5.6 software. RESULTS A total of 61 491 strains of pathogens were isolated from the infectious diseases department patients from Oct 2020 to Sep 2021, 28.8% of which were gram-positive bacteria, and 71.2% were gram-negative bacteria. The isolation rates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MRCNS) were 28.3% and 64.5%, respectively. The drug resistance rates of MRSA and MRCNS were higher than those of the methicillin-sensitive strains to most of the antibiotics. 12.8% of the MRSA strains were resistant to sulfamethoxazole-trimethoprim, and no vancomycin-resistant strains were found. Among the non-meningitis Streptococcus pneumoniae strains, the isolation rates of penicillin-sensitive and penicillin-resistant strains were 94.5% and 1.1%, respectively. The drug resistance rates of Escherichia coli strains to cefotaxime and imipenem were 50.5% and 1.4%, respectively; the drug resistance rates of the Klebsiella pneumoniae strains to cefotaxime and imipenem were 26% and 6.1%, respectively. The drug resistance rates of the Pseudomonas aeruginosa and Acinetobacter baumannii strains to imipenem were 11.8% and 37.2%, respectively. The drug resistance rate of the Haemophilus influenzae strains to ampicillin was 67.9%. CONCLUSION The pathogens that are isolated from the patients of infectious diseases department are highly resistant to the commonly used antibiotics, especially the MRSA and carbapenem-resistant gram-negative bacilli. It is necessary to strengthen the supervision of antibiotics for the patients of infectious diseases department.