Abstract: OBJECTIVE To investigate the current status of management of nosocomial infection in the private medical institutions in Pudong Shanghai. METHODS A questionnaire survey was conducted for the current status of organization and management of nosocomial infection of 200 private medical institutions in Pudong New Area of Shanghai from Mar 3, 2023 to Mar 17, 2023. The major contents of the survey included the construction of management and organization systems, talents equipping and key links of infection prevention. RESULTS Among the 200 private medical institutions, 45 (22.50%) were set up independent hospital infection department, 85 (42.50%) were under the leadership of deans. 76 (38.00%) were taken charge by infection control professionals, the persons in charge of 134 (67.00%) medical institutions were majored in nursing, the annual income of the persons in charge of 99 (49.50%) medical institutions was less than 100,000 yuan. The allocation of professional staff of the infection control departments met the national requirements. 188 (94.00%) private medical institutions were equipped with hand hygiene facilitates, 74 (37.00%) were equipped with nosocomial infection information system, and 173 (86.50%) were equipped with centralized cleaning, disinfection and drying facilities for clean apparatuses. 105 (52.50%) conducted the surveillance of morbidity rates of nosocomial infection, 95 (47.50%) conducted the surveillance of nosocomial infection outbreaks, and 56 (28.00%) conducted the surveillance of multidrug-resistant organisms infections. CONCLUSION There is significant difference in the management mechanism of nosocomial infection among the private medical institutions, the equipped talents are remarkably insufficient in the infection control departments, and the quality of management of nosocomial infection needs to be intensified.